To commemorate their friendship in "The Rothman Disintegration", Amy had commissioned a painting of the two of them, with a confident-looking Amy standing with her left arm draped over the shoulders of an uneasy-looking Penny, which Amy paid $3000 for to give to Penny to honor their friendship. The painting is huge, generally brown in color and considered ugly by most everybody who sees it. Also, Penny's features in the painting are claimed by Bernadette, in her opinion, to be somewhat "masculine". Penny accepts it and with Amy's help, it is mounted on the right side of her entrance doorway. Penny decides to remove it when Amy is not around; however, Amy realizes this and, upset that Penny does not like the painting and assuming that Penny does not value their friendship, takes the painting back. Penny rushes over to Amy's apartment to save their friendship and she takes the painting back. The painting is then remounted next to Penny's entertainment center on the nonexistent wall facing the audience in Apartment 4B and is not seen again until Penny moves her belongings out of her old apartment ("The Property Division Collision"). It gets mounted in what is now Leonard and Penny's apartment again mounted on the nonexistent wall facing the audience next to Leonard's desk. It is also revealed that the artist committed suicide shortly after completing the portrait, which Amy considers will eventually give it considerable resale value.
In "The Property Division Collision", after Sheldon and Amy move into Penny's old apartment, Shamy offers the painting to Leonard and Penny; however, it ends up back in Apartment 4B. After Penny talks to Sheldon about Dr. Ramona Nowitzki's interest in him, she tells the Amy in that painting that she tried to tell Sheldon what was going on in "The Long Distance Dissonance".