Gladys is the aunt of Howard Joel Wolowitz and may be the sister of either Deborah Melvina Wolowitz or her ex-husband Sam Wolowitz. In "The Comic Book Store Regeneration", Debbie is visiting Gladys in Florida and passes away in her sleep which also acknowledges the passing of the actress who played her, Carol Ann Susi. By Howard's marriage, she is the aunt-in-law of Bernadette Rostenkowski-Wolowitz. She is the great-aunt of Howard and Bernadette's daughter Halley Wolowitz and son Neil Michael Wolowitz.
She must have been living in California at the time of "The Prom Equivalency", because Stuart met Howard's cousin Jeannie at her house. Jeannie was the second cousin that Howard lost his virginity to.